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RFP/GFP Safe-Harbor HEK293 Cell Line

數(shù)量:1供應(yīng)商:BPSBioscience細(xì)胞類(lèi)型:穩(wěn)轉(zhuǎn)細(xì)胞系品系:HEK293

  • 產(chǎn)品型號(hào):
  • 廠商性質(zhì):生產(chǎn)廠家
  • 更新時(shí)間:2023-10-12
  • 訪  問(wèn)  量:213
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  • 數(shù)量:

    1

  • 供應(yīng)商:

    BPS Bioscience

  • 細(xì)胞類(lèi)型:

    穩(wěn)轉(zhuǎn)細(xì)胞系

  • 品系:

    HEK293, Human Embryonic Kidney, epithelial-like cells, adherent

  • 組織來(lái)源:

    /

  • 相關(guān)疾?。?/span>

    多種

  • 物種來(lái)源:

    human

  • 免疫類(lèi)型:

    多種

  • 器官來(lái)源:

    無(wú)

  • 運(yùn)輸方式:

    干冰

  • 年限:

    10

  • 生長(zhǎng)狀態(tài):

    良好

  • CAS號(hào):

    無(wú)

  • 英文名:

    RFP/GFP Safe-Harbor HEK293 Cell Line

  • 注冊(cè)證號(hào):

    /

  • 規(guī)格:

    2管

Recombinant stable HEK293 cell line constitutively expressing RFP (Red Fluorescent Protein) and GFP (Green Fluorescent Protein), which have been stably integrated into the AAVS1 safe harbor locus on chromosome 19.Expression of RFP is driven by a CMV promoter, whereas GFP is driven by an EF1A promoter. The combined DNA fragment (CMV-RFP-bGH Poly A-EF1A-GFP-T2A-Puro-SV40 Poly A) (Figure 1) was integrated at the AAVS1 safe harbor locus using CRISPR/Cas9 technology. Cells were cloned by limiting dilution to obtain a monoclonal population. Without the adverse effects resulting from random integrations of RFP-GFP into the HEK293 genome, this cell line behaves like parental HEK293 cells.

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